甘露寡糖和合生素的补充对热应激前后金头鲷的生长性能和免疫反应...

来自 : www.x-mol.com/paper/1372275161 发布时间：2021-03-25

In this study, the effects of mannan oligosaccharides (MOS) and a commercial synbiotic (Biomin)‐supplemented diets on growth and survival performance of gilthead sea bream after eight weeks culture at 27ºC and then ten days at 34ºC were evaluated. Before heat stress, the Biomin supplemented diet significantly increased the number of red blood cells (RBC) and eosinophils of the fish. The MOS‐supplemented diet increased the haematocrit, haemoglobin, lymphocytes, white blood cells (WBC) and monocytes significantly. Total immunoglobulin was significantly higher in both supplemented diets in contrast to the control group. After thermal stress, experimental diets increased the monocyte counts, lysozyme, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of the fish significantly, also the lowest concentration of cortisol was observed in the fish fed with Biomin supplemented diet. Gene expression profile showed a significantly higher expression of innate immune genes (il‐1β, ghr‐1, tnf‐α and igf‐1) in fish fed with Biomin and MOS supplemented diets. However, after thermal challenge, inflammatory response was significantly lower the fish fed with the supplemented diets. According to our results, MOS‐ and Biomin‐supplemented diets enhance the immune health status of the gilthead sea bream during thermal stress.
7天前在这项研究中,评估了甘露寡糖(MOS)和补充商业合生素(Biomin)的饮食对在27ºC培养8周,然后在34ºC培养10天后的金头鲷生长和存活性能的影响。在热应激之...甘露寡糖和合生素的补充对热应激前后金头鲷的生长性能和免疫反应的影响; Kheyrollah Khosravi‐Katuli, Yaghoob Mohammadi, Mehrdad Ranjbaran, Hamed Ghanaatian, Aida Khazaali, Hamed Paknejad, Javier Santander; Aquac. Res.; Aquaculture Research; X-MOL在这项研究中，评估了甘露寡糖（MOS）和补充商业合生素（Biomin）的饮食对在27ºC培养8周，然后在34ºC培养10天后的金头鲷生长和存活性能的影响。在热应激之前，Biomin补充饮食显着增加了鱼的红细胞（RBC）和嗜酸性粒细胞的数量。补充MOS的饮食显着增加了血细胞比容，血红蛋白，淋巴细胞，白细胞（WBC）和单核细胞。与对照组相比，两种补充饮食中的总免疫球蛋白均显着较高。热应激后，实验饮食显着增加了鱼的单核细胞计数，溶菌酶，丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST），在用Biomin补充饲料喂养的鱼中，皮质醇的浓度也最低。基因表达谱显示先天免疫基因的表达明显较高（补充有Biomin和MOS的饲料的鱼类中的il - 1β，ghr - 1，tnf - α和igf - 1）。但是，经过热刺激后，补充饮食喂养的鱼的炎症反应明显降低。根据我们的结果，在热应激期间，以MOS和Biomin补充的饮食可提高金头鲷的免疫健康状况。甘露寡糖和合生素的补充对热应激前后金头鲷的生长性能和免疫反应的影响,Aquaculture Research